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Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

BACKGROUND: Quantitative real-time PCR (qPCR) has become the “gold standard” for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data. RESULTS: Here we undertook a...

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Main Authors: Kagias, Konstantinos, Podolska, Agnieszka, Pocock, Roger
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3997968/
https://ncbi.nlm.nih.gov/pubmed/24656064
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-15-222
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