Application of Qualitative and Quantitative Real-Time PCR, Direct Sequencing, and Terminal Restriction Fragment Length Polymorphism Analysis for Detection and Identification of Polymicrobial 16S rRNA Genes in Ascites

Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml(−1). Direct sequencing of PCR products...

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Detalhes bibliográficos
Main Authors: Krohn, Sandra, Böhm, Stephan, Engelmann, Cornelius, Hartmann, Jan, Brodzinski, Annika, Chatzinotas, Antonis, Zeller, Katharina, Prywerek, Delia, Fetzer, Ingo, Berg, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3993693/
https://ncbi.nlm.nih.gov/pubmed/24622095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00552-14
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