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Genetic Manipulations of the Hyperthermophilic Piezophilic Archaeon Thermococcus barophilus
In this study, we developed a gene disruption system for Thermococcus barophilus using simvastatin for positive selection and 5-fluoroorotic acid (5-FOA) for negative selection or counterselection to obtain markerless deletion mutants using single- and double-crossover events. Disruption plasmids ca...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3993132/ https://ncbi.nlm.nih.gov/pubmed/24487541 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00084-14 |
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