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A Simple and Effective Method for Construction of Escherichia coli Strains Proficient for Genome Engineering

Multiplex genome engineering is a standalone recombineering tool for large-scale programming and accelerated evolution of cells. However, this advanced genome engineering technique has been limited to use in selected bacterial strains. We developed a simple and effective strain-independent method fo...

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Autors principals: Ryu, Young Shin, Biswas, Rajesh Kumar, Shin, Kwangsu, Parisutham, Vinuselvi, Kim, Suk Min, Lee, Sung Kuk
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3991648/
https://ncbi.nlm.nih.gov/pubmed/24747264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094266
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