Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes

The deep sequencing of 16S rRNA genes amplified by universal primers has revolutionized our understanding of microbial communities by allowing the characterization of the diversity of the uncultured majority. However, some universal primers also amplify eukaryotic rRNA genes, leading to a decrease i...

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Main Authors: Mori, Hiroshi, Maruyama, Fumito, Kato, Hiromi, Toyoda, Atsushi, Dozono, Ayumi, Ohtsubo, Yoshiyuki, Nagata, Yuji, Fujiyama, Asao, Tsuda, Masataka, Kurokawa, Ken
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3989492/
https://ncbi.nlm.nih.gov/pubmed/24277737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/dnares/dst052
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