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Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice

Transcranial two-photon microscopy allows long-term imaging of neurons, glia, and vasculature in the intact cortex of living animals. So far, this technique has been primarily used to acquire images in anesthetized animals. Here, we describe a detailed protocol for high-resolution two-photon imaging...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Yang, Guang, Pan, Feng, Chang, Paul C., Gooden, Frank, Gan, Wen-Biao
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3987700/
https://ncbi.nlm.nih.gov/pubmed/23754217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-411-1_3
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