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Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice
Transcranial two-photon microscopy allows long-term imaging of neurons, glia, and vasculature in the intact cortex of living animals. So far, this technique has been primarily used to acquire images in anesthetized animals. Here, we describe a detailed protocol for high-resolution two-photon imaging...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3987700/ https://ncbi.nlm.nih.gov/pubmed/23754217 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-411-1_3 |
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