Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue

Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 µm, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient...

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Bibliografiske detaljer
Main Authors: Ingaramo, Maria, York, Andrew G., Wawrzusin, Peter, Milberg, Oleg, Hong, Amy, Weigert, Roberto, Shroff, Hari, Patterson, George H.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2014
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3986197/
https://ncbi.nlm.nih.gov/pubmed/24706872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1314447111
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