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Probing Tertiary Structure of Proteins Using Single Trp Mutations with Circular Dichroism at Low Temperature
[Image: see text] Trp is the most spectroscopically informative aromatic amino acid of proteins. However, the near-UV circular dichroism (CD) spectrum of Trp is complicated because the intensity and sign of (1)L(a) and (1)L(b) bands vary independently. To resolve vibronic structure and gain site-spe...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical
Society
2014
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3983331/ https://ncbi.nlm.nih.gov/pubmed/24404774 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp4120145 |
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