Rapid single-colony whole-genome sequencing of bacterial pathogens

OBJECTIVES: As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to genera...

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Hlavní autoři: Köser, Claudio U., Fraser, Louise J., Ioannou, Avgousta, Becq, Jennifer, Ellington, Matthew J., Holden, Matthew T. G., Reuter, Sandra, Török, M. Estée, Bentley, Stephen D., Parkhill, Julian, Gormley, Niall A., Smith, Geoffrey P., Peacock, Sharon J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2014
Témata:
Original Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3977605/
https://ncbi.nlm.nih.gov/pubmed/24370932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jac/dkt494
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