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Rapid single-colony whole-genome sequencing of bacterial pathogens

OBJECTIVES: As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to genera...

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Detalhes bibliográficos
Main Authors: Köser, Claudio U., Fraser, Louise J., Ioannou, Avgousta, Becq, Jennifer, Ellington, Matthew J., Holden, Matthew T. G., Reuter, Sandra, Török, M. Estée, Bentley, Stephen D., Parkhill, Julian, Gormley, Niall A., Smith, Geoffrey P., Peacock, Sharon J.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3977605/
https://ncbi.nlm.nih.gov/pubmed/24370932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jac/dkt494
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