A cosmid vector that facilitates restriction enzyme mapping.
We describe the construction and use of a cosmid vector, loric, which is derived from the phage lambda origin of replication and appears to be more stable than ColE1-derived cosmids. Loric recombinants can be efficiently packaged in vivo to yield 100-300 micrograms of DNA per liter that is linear an...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
1985
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397734/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2987911/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.10.3159 |
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