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Challenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library
Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of...
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| Main Authors: | , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
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Shaheed Beheshti University of Medical Sciences
2014
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3977063/ https://ncbi.nlm.nih.gov/pubmed/24711839 |
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