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When One Plus One Does Not Equal Two: Fluorescence Anisotropy in Aggregates and Multiply Labeled Proteins

The behavior of fluorescence anisotropy and polarization in systems with multiple dyes is well known. Homo-FRET and its consequent energy migration cause the fluorescence anisotropy to decrease as the number of like fluorophores within energy transfer distance increases. This behavior is well unders...

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Autors principals: Zolmajd-Haghighi, Zahra, Hanley, Quentin S.
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3976527/
https://ncbi.nlm.nih.gov/pubmed/24703307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.02.020
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