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Cloning of Physarum actin sequences in an exonuclease-deficient bacterial host.
A genomic library of Physarum was constructed in the replacement vector EMBL3. Efficient propagation of the recombinant phages occurred only on the recBC-sbcB- host Escherichia coli CES200, which is deficient in the exonucleases I and V. Thirteen different recombinants with actin-related sequences w...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2986129/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.9.2698 |
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