Overexpression, crystallization and preliminary X-ray crystallographic analysis of the ectoine hydroxylase from Sphingopyxis alaskensis

The ectoine hydroxylase (EctD) is a member of the non-haem-containing iron(II)- and 2-oxoglutarate-dependent dioxygenase superfamily. Its mononuclear iron centre is a prerequisite for the activity of this enzyme and promotes the O(2)-dependent oxidative decarboxylation of 2-oxoglutarate, which is co...

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Bibliographic Details
Main Authors: Hoeppner, Astrid, Widderich, Nils, Bremer, Erhard, Smits, Sander H. J.
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2014
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3976071/
https://ncbi.nlm.nih.gov/pubmed/24699747
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14004798
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