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Crystallization and preliminary X-ray diffraction analysis of domain-chimeric l-(2S,3S)-butanediol dehydrogenase

A domain-chimeric l-2,3-butanediol dehydrogenase (chimera l-BDH), which was designed to possess both the S-configuration specificity of l-BDH and the stability of meso-BDH, was constructed by exchanging the respective domains of these two BDHs. However, chimera l-BDH possessed a lower enzymatic func...

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Bibliografiske detaljer
Main Authors: Shimegi, Tomohito, Ooyama, Takuji, Ohtsuki, Takashi, Kurisu, Genji, Kusunoki, Masami, Ui, Sadaharu
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3976062/
https://ncbi.nlm.nih.gov/pubmed/24699738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X13032755
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