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Construction of telocentric chromosomes in Saccharomyces cerevisiae.
We describe a simple method for the construction of large chromosomal deletions in yeast. Diploid yeast cells were transformed with DNA fragments that replace large regions of the chromosomes by homologous recombination. Using this method, we have constructed a telocentric chromosome III in which ap...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397501/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3885221/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.7.2106 |
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