Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting

Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HR...

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Detalhes bibliográficos
Main Authors: da Silva, Joas Lucas, Leite, Gabriela Guimaraes Sousa, Bastos, Gisele Medeiros, Lucas, Beatriz Cacciacarro, Shinohara, Daniel Keniti, Takinami, Joice Sayuri, Miyata, Marcelo, Fajardo, Cristina Moreno, Luchessi, André Ducati, Leite, Clarice Queico Fujimura, Cardoso, Rosilene Fressatti, Hirata, Rosario Dominguez Crespo, Hirata, Mario Hiroyuki
Formato: Artigo
Idioma:Inglês
Publicado em: Instituto Oswaldo Cruz, Ministério da Saúde 2013
Assuntos:
Short Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3974309/
https://ncbi.nlm.nih.gov/pubmed/23440123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S0074-02762013000100017
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