Full Genome Virus Detection in Fecal Samples Using Sensitive Nucleic Acid Preparation, Deep Sequencing, and a Novel Iterative Sequence Classification Algorithm

We have developed a full genome virus detection process that combines sensitive nucleic acid preparation optimised for virus identification in fecal material with Illumina MiSeq sequencing and a novel post-sequencing virus identification algorithm. Enriched viral nucleic acid was converted to double...

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Detalhes bibliográficos
Main Authors: Cotten, Matthew, Oude Munnink, Bas, Canuti, Marta, Deijs, Martin, Watson, Simon J., Kellam, Paul, van der Hoek, Lia
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973683/
https://ncbi.nlm.nih.gov/pubmed/24695106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0093269
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