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Isolation and Validation of an Endogenous Fluorescent Nucleoid Reporter in Salmonella Typhimurium

In this study we adapted a Mud-based delivery system to construct a random yfp reporter gene (encoding the yellow fluorescent protein) insertion library in the genome of Salmonella Typhimurium LT2, and used fluorescence activated cell sorting and fluorescence microscopy to screen for translational f...

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Detalhes bibliográficos
Main Authors: Passaris, Ioannis, Ghosh, Anirban, Cenens, William, Michiels, Chris W., Lammertyn, Jeroen, Aertsen, Abram
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973593/
https://ncbi.nlm.nih.gov/pubmed/24695782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0093785
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