Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.
A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain...
Gardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397331/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856845/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.6.1658 |
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