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Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker.

A bifunctional fluorescent alkylating agent, dibromobimane, has been used to stabilize a preexisting primary loop in myosin subfragment 1 (S-1). The crosslink achieved joins Cys-707 (called sulfhydryl group "SH1") of the 20-kDa domain (formerly called "20K" domain) with a thiol of the 50-kDa domain...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Mornet, D, Ue, K, Morales, M F
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1985
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397331/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856845/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.6.1658
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