Filter-Aided N-Glycan Separation (FANGS): A Convenient Sample Preparation Method for Mass Spectrometric N-Glycan Profiling

[Image: see text] We have developed a simple method for the release and isolation of glycoprotein N-glycans from whole-cell lysates using less than a million cells, for subsequent implementation with mass spectrometric analysis. Cellular protein extracts prepared using SDS solubilization were sequen...

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Detalhes bibliográficos
Main Authors: Abdul Rahman, Salina, Bergström, Ed, Watson, Christopher J., Wilson, Katherine M., Ashford, David A., Thomas, Jerry R., Ungar, Daniel, Thomas-Oates, Jane E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3971760/
https://ncbi.nlm.nih.gov/pubmed/24450425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr401043r
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