Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II).
The synthesis of a DNA hybridization probe 19 nucleotides in length, equipped with the metal chelator EDTA at C-5 of thymidine in position 10 (indicated by T*) is described. DNA-EDTA 1 has the sequence 5'-T-A-A-C-G-C-A-G-T*-C-A-G-G-C-A-C-C-G-T-3', which is complementary to a 19-nucleotide sequence i...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397174/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3919391/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.4.968 |
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