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Nick-translation of metaphase chromosomes: in vitro labeling of nuclease-hypersensitive regions in chromosomes.

Chinese hamster metaphase chromosomes were labeled by nick-translation, which involved pretreatment of metaphase chromosomes with low levels of DNase I followed by incubation with DNA polymerase I and radioactively labeled nucleotides. The labeled DNA was located on nuclease-hypersensitive regions o...

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Hlavní autoři: Kuo, M T, Plunkett, W
Médium: Artigo
Jazyk:Inglês
Vydáno: 1985
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC397145/
https://ncbi.nlm.nih.gov/pubmed/3856236
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