Nick-translation of metaphase chromosomes: in vitro labeling of nuclease-hypersensitive regions in chromosomes.
Chinese hamster metaphase chromosomes were labeled by nick-translation, which involved pretreatment of metaphase chromosomes with low levels of DNase I followed by incubation with DNA polymerase I and radioactively labeled nucleotides. The labeled DNA was located on nuclease-hypersensitive regions o...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1985
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397145/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856236/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.3.854 |
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