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A cosmid for selecting genes by complementation in Aspergillus nidulans: Selection of the developmentally regulated yA locus

We constructed a 9.9-kilobase cloning vector, designated pKBY2, for isolating genes by complementation of mutations in Aspergillus nidulans. pKBY2 contains the bacteriophage λ cos site, to permit in vitro assembly of phage particles; a bacterial origin of replication and genes for resistance to ampi...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Yelton, M. Melanie, Timberlake, William E., Hondel, C. A. M. J. J. van den
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397141/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593541/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.3.834
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