Optimisation of an asymmetric polymerase chain reaction assay for the amplification of single-stranded DNA from Wuchereria bancrofti for electrochemical detection

Single-stranded DNA (ssDNA) is a prerequisite for electrochemical sensor-based detection of parasite DNA and other diagnostic applications. To achieve this detection, an asymmetric polymerase chain reaction method was optimised. This method facilitates amplification of ssDNA from the human lymphatic...

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Bibliografiske detaljer
Main Authors: Venkatesan, Vasuki, Hoti, Sugeerappa Laxmanappa, Kamaraj, Nagalakshmi, Ghosh, Somnath, Rajaram, Kaushik
Format: Artigo
Sprog:Inglês
Udgivet: Instituto Oswaldo Cruz, Ministério da Saúde 2013
Fag:
Short Communications
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3970695/
https://ncbi.nlm.nih.gov/pubmed/24037206
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/0074-0276108062013020
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