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Purification of the Rous sarcoma virus src kinase by casein-agarose and tyrosine-agarose affinity chromatography.

A simple and effective purification method for the src kinase, the transforming gene product of Rous sarcoma virus, has been developed by using affinity chromatography on casein-agarose and tyrosine-agarose columns. NaDodSO4/polyacrylamide gel electrophoresis and silver staining analysis showed that...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Fukami, Y, Lipmann, F
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397029/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2982151/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.2.321
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