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Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Homogenization by bead beating is a fast and efficient way to release DNA, RNA, proteins, and metabolites from budding yeast cells, which are notoriously hard to disrupt. Here we describe the use of a bead mill homogenizer for the extraction of proteins into buffers optimized to maintain the functio...

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Detalhes bibliográficos
Main Authors: Szymanski, Eva Paige, Kerscher, Oliver
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3968972/
https://ncbi.nlm.nih.gov/pubmed/24300101
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50921
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