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Analysis of Complementarity Requirements for Plant MicroRNA Targeting Using a Nicotiana benthamiana Quantitative Transient Assay

MicroRNAs (miRNAs) guide RNA-induced silencing complexes to target RNAs based on miRNA-target complementarity. Using a dual-luciferase based sensor system in Nicotiana benthamiana, we quantitatively assessed the relationship between miRNA-target complementarity and silencing efficacy measured at bot...

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Detalhes bibliográficos
Main Authors: Liu, Qikun, Wang, Feng, Axtell, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Biologists 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3967037/
https://ncbi.nlm.nih.gov/pubmed/24510721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1105/tpc.113.120972
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