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A Simplified Purification and Some Properties of Ribulose 1,5-Diphosphate Carboxylase from Barley

A rapid procedure was developed for purifying ribulose 1,5-diphosphate carboxylase from barley leaves. After (NH(4))(2)SO(4) fractionation, the unique sedimentation properties of the enzyme were exploited to effect a single step purification to 90% homogeneity. High speed centrifugation pelleted the...

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Main Authors: Kleinkopf, G. E., Huffaker, R. C., Matheson, A.
Formato: Artigo
Idioma:Inglês
Publicado: 1970
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC396563/
https://ncbi.nlm.nih.gov/pubmed/16657435
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