Chemoselective Immobilization of Proteins by Microcontact Printing and Bioorthogonal Click Reactions

Herein, a combination of microcontact printing of functionalized alkanethiols and site-specific modification of proteins is utilized to chemoselectively immobilize proteins onto gold surfaces either by oxime or copper catalyzed alkyne-azide click chemistry. Two molecules capable of click reactions,...

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Detalhes bibliográficos
Main Authors: Tolstyka, Zachary P., Richardson, Wade, Bat, Erhan, Stevens, Caitlin J., Parra, Dayanara P., Dozier, Jonathan K., Distefano, Mark D., Dunn, Bruce, Maynard, Heather D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3962834/
https://ncbi.nlm.nih.gov/pubmed/24166802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201300478
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