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Direct Observation of Unstained Biological Specimens in Water by the Frequency Transmission Electric-Field Method Using SEM

Scanning electron microscopy (SEM) is a powerful tool for the direct visualization of biological specimens at nanometre-scale resolution. However, images of unstained specimens in water using an atmospheric holder exhibit very poor contrast and heavy radiation damage. Here, we present a new form of...

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Bibliographic Details
Main Author: Ogura, Toshihiko
Format: Artigo
Language:Inglês
Published: Public Library of Science 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3961424/
https://ncbi.nlm.nih.gov/pubmed/24651483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0092780
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