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Direct Observation of Unstained Biological Specimens in Water by the Frequency Transmission Electric-Field Method Using SEM

Scanning electron microscopy (SEM) is a powerful tool for the direct visualization of biological specimens at nanometre-scale resolution. However, images of unstained specimens in water using an atmospheric holder exhibit very poor contrast and heavy radiation damage. Here, we present a new form of...

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Detalles Bibliográficos
Autor Principal: Ogura, Toshihiko
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2014
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3961424/
https://ncbi.nlm.nih.gov/pubmed/24651483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0092780
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