Effect of Leukemia Inhibitory Factor and Forskolin on Establishment of Rat Embryonic Stem Cell Lines

This study was designed to investigate whether supplementation of 2i medium with leukemia inhibitory factor (LIF) and/or forskolin would support establishment of germline-competent rat embryonic stem (ES) cell lines. Due to the higher likelihood of outgrowth rates, supplementation of forskolin with...

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Bibliographic Details
Main Authors: HIRABAYASHI, Masumi, GOTO, Teppei, TAMURA, Chihiro, SANBO, Makoto, HARA, Hiromasa, HOCHI, Shinichi
Format: Artigo
Language:Inglês
Published: The Society for Reproduction and Development 2013
Subjects:
Technology Report
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3958585/
https://ncbi.nlm.nih.gov/pubmed/24317016
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1262/jrd.2013-109
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