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An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: Downstream processing and biochemical characterization

An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lip...

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Bibliografiske detaljer
Main Authors: Bisht, Deepali, Yadav, Santosh Kumar, Darmwal, Nandan Singh
Format: Artigo
Sprog:Inglês
Udgivet: Brazilian Society of Microbiology 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3958203/
https://ncbi.nlm.nih.gov/pubmed/24688527
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