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An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: Downstream processing and biochemical characterization

An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lip...

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Detalhes bibliográficos
Main Authors: Bisht, Deepali, Yadav, Santosh Kumar, Darmwal, Nandan Singh
Formato: Artigo
Idioma:Inglês
Publicado em: Brazilian Society of Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3958203/
https://ncbi.nlm.nih.gov/pubmed/24688527
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