Development of a Luciferase Immunoprecipitation System Assay To Detect IgG Antibodies against Human Respiratory Syncytial Virus Nucleoprotein

The nucleoprotein of respiratory syncytial virus (RSV-N) is immunogenic and elicits an IgG response following infection. The RSV-N gene was cloned into a mammalian expression vector, pREN2, and the expressed luciferase-tagged protein (Ruc-N) detected anti-RSV-N-specific IgG antibodies using a high-t...

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Detalhes bibliográficos
Main Authors: Kumari, Sangeeta, Crim, Roberta Lynne, Kulkarni, Ashwin, Audet, Susette A., Mdluli, Thembi, Murata, Haruhiko, Beeler, Judy A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Diagnostic Laboratory Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3957679/
https://ncbi.nlm.nih.gov/pubmed/24403526
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00594-13
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