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Hot-Alkaline DNA Extraction Method for Deep-Subseafloor Archaeal Communities
A prerequisite for DNA-based microbial community analysis is even and effective cell disruption for DNA extraction. With a commonly used DNA extraction kit, roughly two-thirds of subseafloor sediment microbial cells remain intact on average (i.e., the cells are not disrupted), indicating that microb...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3957647/ https://ncbi.nlm.nih.gov/pubmed/24441163 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.04150-13 |
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