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Effect of pH on the Hydrolytic Kinetics of Gamma-Glutamyl Transferase from Bacillus subtilis

The effect of pH on the steady state kinetics of gamma-glutamyl transferase (GGT) from Bacillus subtilis was examined using glutamyl-(3-carboxyl)-4-nitroanilide as the chromogenic reporter substrate. The enzyme was active in the pH range 7.0–11.0 with the optimum activity at pH 11.0. We noticed a pH...

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Detalhes bibliográficos
Main Authors: Balakrishna, Sharath, Prabhune, Asmita
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3956291/
https://ncbi.nlm.nih.gov/pubmed/24719567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/216270
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