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A novel, rapid in cell RNA amplification technique for the detection of low copy mRNA transcripts.

Growing interest now focuses on improvements of in situ polymerase chain reaction (PCR) technology for the detection of DNA and RNA cellular sequences. In this study, reverse transcription PCR in situ hybridisation (RT PCR-ISH) was developed and used to determine gene expression of pyruvate dehydrog...

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Bibliographic Details
Main Authors: Uhlmann, V, Rolfs, A, Mix, E, Silva, I, Hully, J, Lu, L, Lohman, K, Howells, D, Picton, S, O'Leary, J J
Format: Artigo
Language:Inglês
Published: 1998
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC395629/
https://ncbi.nlm.nih.gov/pubmed/9850340
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