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Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment

To investigate the function of a bacterial-type phosphoenolpyruvate carboxylase (PEPC2) derived from photosynthetically-grown Chlamydomonas reinhardtii, a fragment of the pepc2 gene was cloned and expressed in Escherichia coli. After optimal induction for 6 h, PEPC activity in the reverse mutant was...

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Detalhes bibliográficos
Main Authors: Tian, Qi-Lin, Shi, Ding-Ji, Jia, Xiao-Hui, Mi, Hua-Ling, Huang, Xi-Wen, He, Pei-Min
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3955138/
https://ncbi.nlm.nih.gov/pubmed/24375228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-013-1418-9
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