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A Novel Chimeric Avidin with Increased Thermal Stability Using DNA Shuffling

Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombination and selection by a tailored biopanning protoc...

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Hlavní autoři: Taskinen, Barbara, Airenne, Tomi T., Jänis, Janne, Rahikainen, Rolle, Johnson, Mark S., Kulomaa, Markku S., Hytönen, Vesa P.
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2014
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3954883/
https://ncbi.nlm.nih.gov/pubmed/24632863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0092058
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