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Folding of firefly luciferase during translation in a cell-free system.

In vitro synthesis of firefly luciferase and its folding into an enzymatically active conformation were studied in a wheat germ cell-free translation system. A novel method is described by which the enzymatic activity of newly synthesized luciferase can be monitored continuously in the cell-free sys...

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Detalhes bibliográficos
Main Authors: Kolb, V A, Makeyev, E V, Spirin, A S
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC395268/
https://ncbi.nlm.nih.gov/pubmed/8062837
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