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Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters

To monitor nitrate and peptide transport activity in vivo, we converted the dual-affinity nitrate transceptor CHL1/NRT1.1/NPF6.3 and four related oligopeptide transporters PTR1, 2, 4, and 5 into fluorescence activity sensors (NiTrac1, PepTrac). Substrate addition to yeast expressing transporter fusi...

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Detalhes bibliográficos
Main Authors: Ho, Cheng-Hsun, Frommer, Wolf B
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3950950/
https://ncbi.nlm.nih.gov/pubmed/24623305
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.01917
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