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Exploiting Radiation Damage to Map Proteins in Nucleoprotein Complexes: The Internal Structure of Bacteriophage T7

In the final stage of radiation damage in cryo-electron microscopy of proteins, bubbles of hydrogen gas are generated. Proteins embedded in DNA bubble sooner than free-standing proteins and DNA does not bubble under the same conditions. These properties make it possible to distinguish protein from D...

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Autori principali: Cheng, Naiqian, Wu, Weimin, Watts, Norman R., Steven, Alasdair C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3950834/
https://ncbi.nlm.nih.gov/pubmed/24345345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2013.12.004
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