A mutant T7 RNA polymerase as a DNA polymerase.
We have identified a T7 RNA polymerase (RNAP) mutant that efficiently utilizes deoxyribonucleoside triphosphates. In vitro this mutant will synthesize RNA, DNA or 'transcripts' of mixed dNMP/rNMP composition depending on the mix of NTPs present in the synthesis reaction. The mutation is conservative...
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| Опубликовано в:: | EMBO J |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Nature Publishing Group
1995
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394553/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7556104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1995.tb00140.x |
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