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Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transposition.

The Tn10 transposition reaction has been reconstituted in vitro on short linear substrate fragments encoding transposon ends. This permits the direct detection of protein-DNA complexes formed during transposition by gel retardation analysis. We demonstrate that a stable synaptic complex containing t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sakai, J, Chalmers, R M, Kleckner, N
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1995
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC394522/
https://ncbi.nlm.nih.gov/pubmed/7556079
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