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The Saccharomyces cerevisiae translation initiation factor Tif3 and its mammalian homologue, eIF-4B, have RNA annealing activity.

The Saccharomyces cerevisiae TIF3 gene encodes the yeast homologue of mammalian translation initiation factor eIF-4B. We have added six histidine residues to the C-terminus of Tif3 protein (Tif3-His6p) and purified the tagged protein by affinity chromatography. Tif3-His6p stimulates translation and...

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Bibliografiske detaljer
Udgivet i:EMBO J
Principais autores: Altmann, M, Wittmer, B, Méthot, N, Sonenberg, N, Trachsel, H
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 1995
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394456/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7543843/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1995.tb00051.x
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