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Nickel binding properties of Helicobacter pylori UreF, an accessory protein in the nickel-based activation of urease

Helicobacter pylori UreF is involved in the insertion of Ni(2+) in the urease active site. The recombinant protein in solution is a dimer characterized by an extensive α-helical structure and a well-folded tertiary structure. HpUreF binds two Ni(2+) ions per dimer, with micromolar dissociation const...

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Detalhes bibliográficos
Main Authors: Zambelli, Barbara, Berardi, Andrea, Martin-Diaconescu, Vlad, Mazzei, Luca, Musiani, Francesco, Maroney, Michael J., Ciurli, Stefano
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3943961/
https://ncbi.nlm.nih.gov/pubmed/24292245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00775-013-1068-3
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