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Detection of miRNA expression in intact cells using activatable sensor oligonucleotides

We describe a technology for the profiling of miRNA expression in intact cells. The technology is based on sensor oligonucleotides that are cleavable, completely complementary to a target miRNA, and dual-labeled with a fluorescent dye and a quencher. Upon entering the cell, the sensor oligonucleotid...

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Detalhes bibliográficos
Main Authors: Yoo, Byunghee, Kavishwar, Amol, Ghosh, Subrata K, Barteneva, Natalie, Yigit, Mehmet V, Moore, Anna, Medarova, Zdravka
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3943878/
https://ncbi.nlm.nih.gov/pubmed/24440078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2013.12.007
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