Purification of photochemically active halorhodopsin.

We have developed a procedure for the purification of halorhodopsin in a photochemically active state. Solubilization of membranes from a bacteriorhodopsin-negative Halobacterium strain with octyl glucoside was followed by chromatography on hydroxylapatite and octyl-Sepharose gels. All steps were ca...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Taylor, M E, Bogomolni, R A, Weber, H J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1983
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394257/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6578502/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.20.6172
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