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Isolation of amplified DNA sequences from IMR-32 human neuroblastoma cells: facilitation by fluorescence-activated flow sorting of metaphase chromosomes.

Human neuroblastoma IMR-32 cells have large homogeneously staining regions (HSRs), primarily in the short arms of chromosome 1. We have constructed a recombinant phage library that is enriched for DNA present in the HSR of this chromosome by using fluorescence-activated flow sorting for initial chro...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Kanda, N, Schreck, R, Alt, F, Bruns, G, Baltimore, D, Latt, S
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1983
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394202/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6575396/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.13.4069
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